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rabbit polyclonal anti rock  (Proteintech)


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    Proteintech rabbit polyclonal anti rock
    Rabbit Polyclonal Anti Rock, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 120 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal anti rock/product/Proteintech
    Average 95 stars, based on 120 article reviews
    rabbit polyclonal anti rock - by Bioz Stars, 2026-06
    95/100 stars

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    Co-localization of AnxA6 with ROCK in Saos-2 cells, non-treated or treated with different inhibitors, in resting conditions ( A ) or after seven-day stimulation with AA and β-GP ( B ). The cells were incubated with appropriate antibodies: mouse monoclonal anti-AnxA6 linked with goat-anti mouse IgG-FITC and rabbit polyclonal anti-ROCK 2 linked with goat anti-rabbit IgG-TRITC and observed under an Axio Observer Z1 fluorescent microscope (Carl Zeiss, Oberkochen, Germany) with phase contrast and appropriate fluorescent filters, magnification 240×. The yellow color and arrowheads on the merge images indicate protein co-localization. Data of a typical experiment are presented.

    Journal: International Journal of Molecular Sciences

    Article Title: Src and ROCK Kinases Differentially Regulate Mineralization of Human Osteosarcoma Saos-2 Cells

    doi: 10.3390/ijms20122872

    Figure Lengend Snippet: Co-localization of AnxA6 with ROCK in Saos-2 cells, non-treated or treated with different inhibitors, in resting conditions ( A ) or after seven-day stimulation with AA and β-GP ( B ). The cells were incubated with appropriate antibodies: mouse monoclonal anti-AnxA6 linked with goat-anti mouse IgG-FITC and rabbit polyclonal anti-ROCK 2 linked with goat anti-rabbit IgG-TRITC and observed under an Axio Observer Z1 fluorescent microscope (Carl Zeiss, Oberkochen, Germany) with phase contrast and appropriate fluorescent filters, magnification 240×. The yellow color and arrowheads on the merge images indicate protein co-localization. Data of a typical experiment are presented.

    Article Snippet: Then, cells were incubated with mouse monoclonal anti-annexin A6 (AnxA6; 1:100, v:v ; Transduction Laboratories), rabbit polyclonal anti-vinculin (vinculin; 1:100, v:v ; Sigma), rabbit polyclonal anti-non-muscle myosin IIA (MIIA; 1:100, v:v ; Sigma), rabbit polyclonal anti-Src kinase (Src; 1:100, v:v ; Sigma), or rabbit polyclonal anti-ROCK 2 kinase (ROCK; 1:100, v:v ; Sigma) primary antibodies diluted in TTBS containing 0.5% ( w:v ) FBS.

    Techniques: Incubation, Microscopy